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532nm picosecond laser and TRFM

(Summary description)

532nm picosecond laser and TRFM

(Summary description)

Information

Introduction to time-resolved fluorescence:

Time-resolved fluorescence refers to a transient fluorescence spectrum, which is the fluorescence reflection measured at different delay times of the excitation light pulse after the excitation light pulse is cut off, and reflects the movement process of the excited electrons (i.e., fluorescence dynamics).Unlike the steady-state fluorescence, the transient fluorescence mainly obtains the relaxation time of the sample from the excited state to the steady-state through the pulse light source and the fast detector, which is the life of the sample.However, it is basically consistent with the steady-state fluorescence on the optical path.

Test method:

At present, pulse method is the most commonly used technique in the detection of time-resolved fluorescence, among which single photon counting method (TCSPC) is more widely used.The following figure 1, the principle is to use a synchronous signal source driving laser, emergent light pulses irradiation sample pool, taking advantage of photon detection device (mostly PMT) the fluorescence signal detection, each photon counting signal in the counter will fall into a corresponding time window, after hundreds of thousands of times to repeat, different time channel accumulated number of photons.Thus, the distribution probability curve of fluorescence emission photon on the time axis is constructed, that is, the fluorescence life curve (figure 2).

 

 

Test method:

At present, pulse method is the most commonly used technique in the detection of time-resolved fluorescence, among which single photon counting method (TCSPC) is more widely used.The following figure 1, the principle is to use a synchronous signal source driving laser, emergent light pulses irradiation sample pool, taking advantage of photon detection device (mostly PMT) the fluorescence signal detection, each photon counting signal in the counter will fall into a corresponding time window, after hundreds of thousands of times to repeat, different time channel accumulated number of photons.Thus, the distribution probability curve of fluorescence emission photon on the time axis is constructed, that is, the fluorescence life curve (figure 2).

Pulse light source recommended:

Single photon counting (TCSPC) USES statistical methods to calculate the time difference between the first (and only) photon emitted by a sample after excitation and the excited light.Due to the requirement of the luminous time signal, TCSPC generally requires the light source with high repetition frequency as the excitation source, whose repetition should be at least above KHz, and most light sources will reach the MHz level.At the same time, in general, it is necessary to control the number of signals at the time of luminescence, so as to satisfy the effective fluorescence signal with only one photon produced by the sample within one excitation cycle, so as to avoid the appearance of photon pairs.

NPI laser according to the requirements the direction with independent research and development of high frequency mode-locked laser Rainbow 532  nm OEM, its average output power is greater than 20 mw, pulse width is less than 15 ps, weighing up to MHz frequency magnitude, and can do frequency reduction processing according to the needs of the user's system, realize super short pulse and high frequency requirements, perfect correspondence TCSPC research the needs of the user.In addition, the product adopts all-optical fiber structure and back-end frequency doubling module. Its optical path and circuit structure are highly integrated, compact and portable, and convenient for users to realize system miniaturization.

 

Applications:

Wang Xiaomu research group of Nanjing University

 

 

 

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